Matthias Gimpel
Technische Universität Berlin
H-index: 13
Europe-Germany
Description
Matthias Gimpel, With an exceptional h-index of 13 and a recent h-index of 12 (since 2020), a distinguished researcher at Technische Universität Berlin, specializes in the field of gene regulation, strain engineering, regulatory small RNA.
His recent articles reflect a diverse array of research interests and contributions to the field:
Recent insights into the world of dual‐function bacterial sRNAs
Improved preculture management for Cupriavidus necator cultivations
Implementation of a high cell density fed-batch for heterologous production of active [NiFe]-hydrogenase in Escherichia coli bioreactor cultivations
High-Yield Production of Catalytically Active Regulatory [NiFe]-Hydrogenase From Cupriavidus necator in Escherichia coli
Production of soluble regulatory hydrogenase from Ralstonia eutropha in Escherichia coli using a fed-batch-based autoinduction system
Optimization of Culture Conditions for Oxygen-Tolerant Regulatory [NiFe]-Hydrogenase Production from Ralstonia eutropha H16 in Escherichia coli
Heterologous hydrogenase overproduction systems for biotechnology—an overview
General principles for yield optimization of nucleoside phosphorylase‐catalyzed transglycosylations
Professor Information
University | Technische Universität Berlin |
---|---|
Position | Institute of Biotechnology |
Citations(all) | 689 |
Citations(since 2020) | 545 |
Cited By | 290 |
hIndex(all) | 13 |
hIndex(since 2020) | 12 |
i10Index(all) | 13 |
i10Index(since 2020) | 13 |
University Profile Page | Technische Universität Berlin |
Research & Interests List
gene regulation
strain engineering
regulatory small RNA
Top articles of Matthias Gimpel
Recent insights into the world of dual‐function bacterial sRNAs
Dual‐function sRNAs refer to a small subgroup of small regulatory RNAs that merges base‐pairing properties of antisense RNAs with peptide‐encoding properties of mRNA. Both functions can be part of either same or in another metabolic pathway. Here, we want to update the knowledge of to the already known dual‐function sRNAs and review the six new sRNAs found since 2017 regarding their structure, functional mechanisms, evolutionary conservation, and role in the regulation of distinct biological/physiological processes. The increasing identification of dual‐function sRNAs through bioinformatics approaches, RNomics and RNA‐sequencing and the associated increase in regulatory understanding will likely continue to increase at the same rate in the future. This may improve our understanding of the physiology, virulence and resistance of bacteria, as well as enable their use in technical applications. This …
Authors
Sebastian Benjamin Schnoor,Peter Neubauer,Matthias Gimpel
Published Date
2024/1
Improved preculture management for Cupriavidus necator cultivations
ObjectivesResearch on hydrogenases from Cupriavidus necator has been ongoing for more than two decades and still today the common methods for culture inoculation are used. These methods were never adapted to the requirements of modified bacterial strains, resulting in different physiological states of the bacteria in the precultures, which in turn lead prolonged and different lag-phases.ResultsIn order to obtain uniform and always equally fit precultures for inoculation, we have established in this study an optimized protocol for precultures of the derivative of C. necator HF210 (C. necator HP80) which is used for homologous overexpression of the genes for the NAD+-reducing soluble hydrogenase (SH). We compared different media for preculture growth and determined the optimal time point for harvest. The protocol obtained in this study is based on two subsequent precultures, the first one in complex nutrient …
Authors
Michelle-Sophie Gerlach,Peter Neubauer,Matthias Gimpel
Journal
Biotechnology Letters
Published Date
2023/12
Implementation of a high cell density fed-batch for heterologous production of active [NiFe]-hydrogenase in Escherichia coli bioreactor cultivations
BackgroundO2-tolerant [NiFe]-hydrogenases offer tremendous potential for applications in H2-based technology. As these metalloenzymes undergo a complicated maturation process that requires a dedicated set of multiple accessory proteins, their heterologous production is challenging, thus hindering their fundamental understanding and the development of related applications. Taking these challenges into account, we selected the comparably simple regulatory [NiFe]-hydrogenase (RH) from Cupriavidus necator as a model for the development of bioprocesses for heterologous [NiFe]-hydrogenase production. We already reported recently on the high-yield production of catalytically active RH in Escherichia coli by optimizing the culture conditions in shake flasks.ResultsIn this study, we further increase the RH yield and ensure consistent product quality by a rationally designed high cell density fed-batch …
Authors
Qin Fan,Saskia Waldburger,Peter Neubauer,Sebastian L Riedel,Matthias Gimpel
Journal
Microbial cell factories
Published Date
2022/9/19
High-Yield Production of Catalytically Active Regulatory [NiFe]-Hydrogenase From Cupriavidus necator in Escherichia coli
Hydrogenases are biotechnologically relevant metalloenzymes that catalyze the reversible conversion of molecular hydrogen into protons and electrons. The O2-tolerant [NiFe]-hydrogenases from Cupriavidus necator (formerly Ralstonia eutropha) are of particular interest as they maintain catalysis even in the presence of molecular oxygen. However, to meet the demands of biotechnological applications and scientific research, a heterologous production strategy is required to overcome the low production yields in their native host. We have previously used the regulatory hydrogenase (RH) from C. necator as a model for the development of such a heterologous hydrogenase production process in E. coli. Although high protein yields were obtained, the purified enzyme was inactive due to the lack of the catalytic center, which contains an inorganic nickel-iron cofactor. In the present study, we significantly improved the production process to obtain catalytically active RH. We optimized important factors such as O2 content, metal availability, production temperature and time as well as the co-expression of RH-specific maturase genes. The RH was successfully matured during aerobic cultivation of E. coli by co-production of seven hydrogenase-specific maturases and a nickel permease, which was confirmed by activity measurements and spectroscopic investigations of the purified enzyme. The improved production conditions resulted in a high yield of about 80 mg L–1 of catalytically active RH and an up to 160-fold space-time yield in E. coli compared to that in the native host C. necator [<0.1 U (L d) –1]. Our strategy has important implications for the use …
Authors
Qin Fan,Giorgio Caserta,Christian Lorent,Ingo Zebger,Peter Neubauer,Oliver Lenz,Matthias Gimpel
Journal
Frontiers in Microbiology
Published Date
2022/4/29
Production of soluble regulatory hydrogenase from Ralstonia eutropha in Escherichia coli using a fed-batch-based autoinduction system
Background Autoinduction systems can regulate protein production in Escherichia coli without the need to monitor cell growth or add inducer at the proper time following culture growth. Compared to classical IPTG induction, autoinduction provides a simple and fast way to obtain high protein yields. In the present study, we report on the optimization process for the enhanced heterologous production of the Ralstonia eutropha regulatory hydrogenase (RH) in E. coli using autoinduction. These autoinduction methods were combined with the EnPresso B fed-batch like growth system, which applies slow in situ enzymatic glucose release from a polymer to control cell growth and protein synthesis rate. Results We were able to produce 125 mg L−1 RH corresponding to a productivity averaged over the whole process time of 3 mg (L h)−1 in shake flasks using …
Authors
Qin Fan,Peter Neubauer,Matthias Gimpel
Journal
Microbial Cell Factories
Published Date
2021/12
Optimization of Culture Conditions for Oxygen-Tolerant Regulatory [NiFe]-Hydrogenase Production from Ralstonia eutropha H16 in Escherichia coli
Hydrogenases are abundant metalloenzymes that catalyze the reversible conversion of molecular H2 into protons and electrons. Important achievements have been made over the past two decades in the understanding of these highly complex enzymes. However, most hydrogenases have low production yields requiring many efforts and high costs for cultivation limiting their investigation. Heterologous production of these hydrogenases in a robust and genetically tractable expression host is an attractive strategy to make these enzymes more accessible. In the present study, we chose the oxygen-tolerant H2-sensing regulatory [NiFe]-hydrogenase (RH) from Ralstonia eutropha H16 owing to its relatively simple architecture compared to other [NiFe]-hydrogenases as a model to develop a heterologous hydrogenase production system in Escherichia coli. Using screening experiments in 24 deep-well plates with 3 mL working volume, we investigated relevant cultivation parameters, including inducer concentration, expression temperature, and expression time. The RH yield could be increased from 14 mg/L up to >250 mg/L by switching from a batch to an EnPresso B-based fed-batch like cultivation in shake flasks. This yield exceeds the amount of RH purified from the homologous host R. eutropha by several 100-fold. Additionally, we report the successful overproduction of the RH single subunits HoxB and HoxC, suitable for biochemical and spectroscopic investigations. Even though both RH and HoxC proteins were isolated in an inactive, cofactor free apo-form, the proposed strategy may powerfully accelerate bioprocess development and …
Authors
Qin Fan,Giorgio Caserta,Christian Lorent,Oliver Lenz,Peter Neubauer,Matthias Gimpel
Journal
Microorganisms
Published Date
2021/5/31
Heterologous hydrogenase overproduction systems for biotechnology—an overview
Hydrogenases are complex metalloenzymes, showing tremendous potential as H2-converting redox catalysts for application in light-driven H2 production, enzymatic fuel cells and H2-driven cofactor regeneration. They catalyze the reversible oxidation of hydrogen into protons and electrons. The apo-enzymes are not active unless they are modified by a complicated post-translational maturation process that is responsible for the assembly and incorporation of the complex metal center. The catalytic center is usually easily inactivated by oxidation, and the separation and purification of the active protein is challenging. The understanding of the catalytic mechanisms progresses slowly, since the purification of the enzymes from their native hosts is often difficult, and in some case impossible. Over the past decades, only a limited number of studies report the homologous or heterologous production of high yields of hydrogenase. In this review, we emphasize recent discoveries that have greatly improved our understanding of microbial hydrogenases. We compare various heterologous hydrogenase production systems as well as in vitro hydrogenase maturation systems and discuss their perspectives for enhanced biohydrogen production. Additionally, activities of hydrogenases isolated from either recombinant organisms or in vivo/in vitro maturation approaches were systematically compared, and future perspectives for this research area are discussed.
Authors
Qin Fan,Peter Neubauer,Oliver Lenz,Matthias Gimpel
Published Date
2020/8/16
General principles for yield optimization of nucleoside phosphorylase‐catalyzed transglycosylations
The biocatalytic synthesis of natural and modified nucleosides with nucleoside phosphorylases offers the protecting‐group‐free direct glycosylation of free nucleobases in transglycosylation reactions. This contribution presents guiding principles for nucleoside phosphorylase‐mediated transglycosylations alongside mathematical tools for straightforward yield optimization. We illustrate how product yields in these reactions can easily be estimated and optimized using the equilibrium constants of phosphorolysis of the nucleosides involved. Furthermore, the varying negative effects of phosphate on transglycosylation yields are demonstrated theoretically and experimentally with several examples. Practical considerations for these reactions from a synthetic perspective are presented, as well as freely available tools that serve to facilitate a reliable choice of reaction conditions to achieve maximum product yields in …
Authors
Felix Kaspar,Robert T Giessmann,Katja F Hellendahl,Peter Neubauer,Anke Wagner,Matthias Gimpel
Journal
ChemBioChem
Published Date
2020/5/15
Professor FAQs
What is Matthias Gimpel's h-index at Technische Universität Berlin?
The h-index of Matthias Gimpel has been 12 since 2020 and 13 in total.
What are Matthias Gimpel's top articles?
The articles with the titles of
Recent insights into the world of dual‐function bacterial sRNAs
Improved preculture management for Cupriavidus necator cultivations
Implementation of a high cell density fed-batch for heterologous production of active [NiFe]-hydrogenase in Escherichia coli bioreactor cultivations
High-Yield Production of Catalytically Active Regulatory [NiFe]-Hydrogenase From Cupriavidus necator in Escherichia coli
Production of soluble regulatory hydrogenase from Ralstonia eutropha in Escherichia coli using a fed-batch-based autoinduction system
Optimization of Culture Conditions for Oxygen-Tolerant Regulatory [NiFe]-Hydrogenase Production from Ralstonia eutropha H16 in Escherichia coli
Heterologous hydrogenase overproduction systems for biotechnology—an overview
General principles for yield optimization of nucleoside phosphorylase‐catalyzed transglycosylations
...
are the top articles of Matthias Gimpel at Technische Universität Berlin.
What are Matthias Gimpel's research interests?
The research interests of Matthias Gimpel are: gene regulation, strain engineering, regulatory small RNA
What is Matthias Gimpel's total number of citations?
Matthias Gimpel has 689 citations in total.